How to Keep Your Equipment from Turning into Lemons by Takashi Yogi Even the best equipment can fail if not treated properly. Here are some suggestions: 1. Keep the equipment clean. Sweep the floors regularly to keep dirt from being sucked into equipment cooling ducts. Drain water baths whenever the water is dirty and refill with DI water only. Change vacuum pump oil frequently. (If the oil looks like chocolate milk, it's very dirty.) Wash and dry centrifuge rotors after use. Wipe up spills immediately-- buffers and salts are corrosive and solvents can ruin plastics. Even DI water can ruin electronic circuits; pull the plug immediately. Cover microscopes. Don't use adhesive tags on centrifuge tubes; they fly off and clog the motor. 2. Be kind to your equipment. Don't pull on electrical cords and gel wires; always pull on the connector. Don't turn the timer knob on Eppendorf centrifuges backwards. Don't drag ultracentrifuge rotors across the table; the speed sensor disk is on the bottom. Always balance centrifuge rotors. Don't try to stop centrifuge rotors by hand; the rotors get damaged and you may too. 3. Call me at the first sign of trouble. Don't wait until a noisy centrifuge finally quits with a ruined motor. I am more than happy to fix the slightest problem like a loose knob or a squeaky hinge. A loose power switch can short circuit and cause major problems. Get worn power cords replaced. If a fuse blows, there is probably a good reason. Unless you are sure of the cause, call me instead of replacing it yourself. If you do replace a fuse make sure that the current, voltage, and time lag rating are the same. The wrong fuse can damage the equipment or cause a fire. 4. Turn off equipment whenever possible unless the instrument requires a long time to stabilize. Turn off expensive UV lamps in spectrophotometers unless you are using them. If a video monitor doesn't have a screen saver, turn it off or dim the screen when not used for long periods.